Job description
Job Summary:
Under general supervision, provides routine technical assistance in performing assigned and delegated tasks in support of research projects.
Job Duties:
1. Molecular Biology: site directed mutagenesis, sequencing and PCR/qRT-PCR. Some training will be provided as necessary. (15%)
2. Biochemical Procedures - Western Blots, immunoblots, SDS-PAGE, quantifying protein concentrations. Some training will be provided as necessary. (20%)
3. General Laboratory Maintenance - preparing agar plates, autoclaving of glassware, media and pipette tips, re-filling purified water and liquid nitrogen dewar. Maintenance of tissue-culture room, incubators, and sterile tissue-culture hoods. Ordering and receiving. (25%)
4. Preparation of primary neurons for culture from rodent peripheral ganglia. Maintenance of tissue-culture cells in tissue-culture room using sterile technique. Transfection of tissue-culture cells. Use of a fluorescent microscope. Animal husbandry. (40%)
Education:
Bachelors in a basic science is required.
Experience:
None
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